Thrombin Time (Abnormals reflex to 1:1 mix)
| Profile or Test Panel |
Abnormal PT or APTT Reflexive Evaluation Profile
Additional assays will be performed as indicated.
Fibrinogen Evaluation Profile
Fibrinolysis Evaluation Profile
Lupus Anticoagulant Profile
Abnormal result reflexes to the following tests as indicated.
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| Method | Clottable |
| Test Information | A low concentration of thrombin is added to plasma and the rate of reaction for the conversion of fibrinogen to fibrin is measured in seconds. The enzymatic cleavage of fibrin peptides is rate limiting because of the low concentration of thrombin. Prolongation of thrombin time can be caused by heparin, elevated levels of fibrin/fibrinogen degradation products (FDP), hereditary or acquired fibrinogen abnormalities (hypofibrinogenemia or dysfibrinogenemia), or the presence of fibrinolytic agents (e.g., tPA, urokinase). |
| Additional Test Information | Normal Range: 18.0 – 24.0 sec |
| Sample Requirements | Whole Blood: 3.2% Sodium Citrated (blue top) OR Frozen Plasma |
| Requested Volume | Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood OR Frozen plasma in 4 X 1mL aliquots in plastic tubes |
| Sample Information | Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection. Frozen Plasma: send on dry iceSend samples to:Bloodworks Northwest Attn: Hemostasis Laboratory 921 Terry Ave Seattle, WA 98104 For additional information, please see Ordering Information |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3200-02 |
| CPT Codes | 85670 |
| Turn around Time (analytic time) | 5 Business Days |
| Report/ Results | Fax number required to receive report |