DNA Factor V Leiden (APC Resistance) Mutation
| Profile or Test Panel |
Thrombosis Genetic Risk Profile
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| Also Known as (Alias) | FV Leiden genotyping |
| Method | polymerase chain reaction (PCR) based |
| Test Information | Factor V Leiden Mutation (hereditary APC Resistance; Factor V “Leiden”, Dahlback, 1995) is a single point mutation of guanine (G) to adenine (A) at position 1691 of the factor V gene. This results in a substitution of glutamine for arginine at amino acid 506 resulting in Factor V Leiden and rendering it partially resistant to inactivation by APC. Their relative risks of venous thrombosis (vs. control) are about 7- and 80-fold (Rosendaal et al., 1995). |
| Additional Test Information | Test Values: Homozygous glutamine (Gin506) Heterozygous or Homozygous arginine (Arg506) abnormal values *Note abnormal tests are confirmed by repeating tests. |
| Sample Requirements | EDTA (lavender top) |
| Requested Volume | 5cc EDTA (lavender top) tube |
| Minimum Volume or Pediatric volume | 5cc EDTA (lavender top) tube |
| Shipping Information | Whole blood shipped at ambient temperature and should arrive in the lab within 48 hours.
Send samples to: |
| Requisition Form | Request for Testing Hemostasis Reference Laboratory |
| Transaction Code | 3250-03 |
| CPT Codes | 81241 |
| Test Schedule | Weekly on Tuesdays on samples received Wednesday through Monday noon |
| Turn around Time (analytic time) | 3 business days |
| Report/Results | Fax number required to receive report |