Bloodworks Northwest Laboratories

Initial Evaluation of Recipient and Potential Donor(s) for Living Renal Donor Transplant

When a kidney transplant candidate has a medically-suitable potential living donor(s), it can be a willing family member(s) or friend(s), a series of tests are performed to determine potential compatibility.

The patient and potential donor(s) should be scheduled for initial testing.

The donor(s) is/are ABO typed to confirm compatibility to the patient.  If compatible, the patient’s serum is crossmatched with lymphocytes from the potential donor(s) and from the patient. If the crossmatch is negative, then the donor(s) is/are HLA typed.

Recipient Transaction Code and Test:

• 3103-00 – ABO & Rh typing (for new patients only)
• 3084-06 – HLA Class I + II (A,B,C,DR,DQ) Typing (for new patients only)
• 3088-00 Lymphocyte Crossmatch

Donor(s) Transaction Code and Test

• 3103-00 – ABO & Rh typing (for new donors only)
• 3103-00 – Solid Organ Donor ABO & Rh (includes A1 lectin if group A)
• 3084-06 – HLA Class I + II (A,B,C,DR,DQ) Typing (if lymphocyte crossmatch is negative or per request of transplant center)

Initial Evaluation of Recipient and Donor for a Donor Exchange

The recipient typically has either ABO-incompatible or crossmatch-positive living donor(s); no additional testing required for the recipient at initial evaluation.

Donor(s) Transaction Code and Test

• 3103-00 – Solid Organ Donor ABO & Rh (includes A1 lectin if group A)
• 3084-06 – HLA Class I + II (A,B,C,DR,DQ) Typing
• 3915-21 – High Resolution Typing HLA-DPB1
• 3915-30 – High Resolution Typing HLA-DQA1

Testing Details for an Initial Evaluation Workup

• Specimen Requirements for Patient: Three to five 10cc ACD tubes (yellow top, solution A or B), and One to two 10cc clot tubes (red top)
• Specimen Requirements for Donor: Three 10cc ACD blood (yellow top, solution A or B).
• Labeling Requirements: Specimen must have the patient’s first and last name, a date of draw and a unique identifier written on the tube.
• All specimens must be accompanied with  a Request for Testing Form
• Analytic Time: 5 to 10 business days
• Testing Schedule:   Monday through Friday