Red Cell Genotyping for multiple blood groups
| Also Known as (Alias) | red cell genotyping |
| Indications | Patients with pan reactive autoantibody and/or multiple alloantibodies or multiply transfused can be typed for a predicted phenotype using genetic testing. This is also useful for patients who have a disorder that requires chronic transfusions such as with sickle cell disease. It has been recommended that red cell genotyping be performed before patients begin the anti-CD38 drug daratumumab. |
| Method | polymerase chain reaction (PCR) based |
| Test Information | SNP (single nucleotide polymorphism) genetic testing This includes common variants and extended antigen typing. |
| Sample Requirements | EDTA (lavender top) |
| Requested Volume | 10cc EDTA (lavender top) tube(s) |
| Minimum Volume or Pediatric volume | Minimum Vol: 5ml
Pediatric Minimum Vol: 2ml or 4 EDTA (lavender) microtainers, each containing 0.5ml *Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at 206-689-6269 for approval of microtainers |
| Shipping Information | Whole blood shipped at ambient temperature and should arrive in the lab within 48 hours.
Send samples to: For additional information please see Ordering Information |
| Requisition Form | Request for Testing Genomics Testing Laboratory |
| Transaction Code | 3117-04 |
| CPT Codes | 81403 x33 |
| Turn around Time (analytic time) | 3 business days |
| Report/Results | Fax number required to receive report |
| Limitation of test | Molecular testing to predict the red blood cell phenotype does not always reflect the phenotypic expression of the blood group antigen. This may occur in cases where the individual has genetic variation that silences or alters the blood group antigen expression that is not included in the target region of the test, and also may occur with RhD zygosity testing. Test results must be interpreted with knowledge of how known polymorphisms affect blood group antigen expression. The performance characteristics have been established by Bloodworks Northwest. The results are not intended as the sole means for clinical diagnosis for donor or patient management decisions. |