Indirect Antiglobulin Test (antibody screen)
| Also Known as (Alias) | Antibody screen |
| Indications | This test detects the presence of unexpected antibody (allo- or auto-) against red blood cell antigens. |
| Method | Capture solid phase technology or gel column. |
| Test Information | Patient serum/plasma is tested against a three cell panel that contains 18 common red cell antigens. The majority of clinically significant red blood cell alloantibodies are detected by this test. If positive, a limited cell panel (antibody identification) will be performed to determine red blood cell antigen specificity. If unresolved, referred to IRL. |
| Sample Requirements | EDTA or plain red top (clotted) tube, separator gel tube is not acceptable. |
| Requested Volume | 1 full 7 ml EDTA/clotted sample |
| Minimum Volume or Pediatric volume | Minimum volume: 1 full 7 ml EDTA/clotted sample Pediatric: 1 full 3 ml EDTA; neonate: 2 full 0.5 ml EDTA microtainers |
| Shipping Information | Ship at ambient temperature
Send samples to: |
| Requisition Form | RFT-Request for Testing Transfusion Services |
| Transaction Code | 3104-00 |
| CPT Codes | 86850 |
| Test Schedule | Monday through Sunday |
| Turn around Time (analytic time) | 24 hours if weekday; 48 hours if weekend |
| Report/ Results | Fax number required to receive report |