Factor V Activity
| Profile or Test Panel |
Extrinsic Factor (abnormal PT) Evaluation Profile
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| Method | Clottable |
| Test Information | A one-stage extrinsic system clotting assay is used to measure the factor V level. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Levels are reduced by hereditary or acquired factor V deficiency, factor V inhibitor (including those seen after use of topical bovine thrombin), liver disease, or disseminated intravascular coagulation (DIC). The factor V (Leiden) mutation associated with activated protein C resistance does not affect factor V activity and is tested by separate kinetic or DNA testing (described later). |
| Additional Test Information | Normal Range 78-131% effective 11/1/11 |
| Sample Requirements | Whole Blood: 3.2% Sodium Citrated (blue top) OR Frozen Plasma |
| Requested Volume | Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood OR Frozen plasma in 4 X 1mL aliquots in plastic tubes |
| Minimum Volume or Pediatric volume | Frozen Plasma: at least 0.5ml in 2 plastic tubes. |
| Sample Information | Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection. Frozen Plasma: send on dry iceSend samples to: Bloodworks Northwest For additional information, please see Ordering Information |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3210-11 |
| CPT Codes | 85220 |
| Test Schedule | Monday – Friday |
| Turn around Time (analytic time) | 1 Business Day |
| Report/ Results | Fax number required to receive report |